dspe peg amine  (Croda International Plc)

Journal: Materials Horizons

Article Title: The paradox of gold–liposome nanohybrids: the location of gold governs unconventional properties and drives cellular behavior

doi: 10.1039/d5mh02229k

Figure Lengend Snippet: Synthesis approaches that failed to control opto-mechanical properties of gold–liposome nanohybrids. (a) Schematic representation of gold–liposome nanoparticle synthesis with DPPC/chol/DSPE–PEG/DSPE–PEG–SH liposomes. (b) Extinction spectra of the nanoparticles showing LSPR in the visible region. (c) Hydrodynamic diameter measured using dynamic light scattering, (d) zeta potential showing the surface charge of the different samples. (e) Representative TEM micrograph of the sample with a liposome : gold seed ratio of 1 : 2 (inset: the optical image of the sample in a cuvette). (f) Schematic representation of gold–liposome nanoparticle synthesis using DPPC/chol/DSPE–PEG/DSPE–PEG–NH 2 liposomes. (g) Extinction spectra of the nanoparticles showing LSPR in the visible region. (h) Hydrodynamic diameter and (i) surface charge of the two samples with different ascorbic acid : HAuCl 4 ratios. (j) Representative TEM micrograph of the sample with an ascorbic acid : HAuCl 4 ratio of 8 : 1 (inset: the optical image of the sample in a cuvette). (k) Schematic representation of gold–liposome nanoparticle synthesis using DPPC/chol/DSPE–PEG liposomes by direct reduction of Au with ascorbic acid (AA). (l) Extinction spectra of the nanoparticles showing LSPR of two different ascorbic acid : HAuCl 4 ratios. (m) Hydrodynamic diameter and ( n ) surface charge of the samples ( n = 3 samples per group). (o) Representative TEM micrograph of the sample with an ascorbic acid : HAuCl 4 ratio of 8 : 1 (inset: the optical image of samples in a cuvette). Scale bar = 200 nm. Here, * represents a p value <0.05, ** represents a p value <0.01 and, *** represents a p value <0.001 and ns stands for not significant.

Article Snippet: 1,2-Distearoyl- sn-glycero -3-phosphoethanolamine- N -[methoxy (polyethylene glycol)-2000] (DSPE–PEG), 1,2-dipalmitoyl- sn-glycero -3-phosphocholine (DPPC), and (1,2-distearoyl- sn-glycero -3-phosphoethanolamine-poly(ethylene glycol)-amine) (DSPE–PEG–NH 2 ) were purchased from Avanti Polar Lipids (Alabaster, AL).

Techniques: Control, Liposomes, Zeta Potential Analyzer

Journal: Theranostics

Article Title: Dual-ligand curcin-loaded hybrid solid lipid nanoparticles achieve durable gliosarcoma remission while preserving neuro-behavioral function

doi: 10.7150/thno.123534

Figure Lengend Snippet: Synthesis, physicochemical characterization, and pH-responsive release profile of curcin-loaded dual-targeted hybrid solid lipid nanoparticles (HSLNs). (A) Schematic illustration of lipid components-DSPE-PEG(2000)-amine, phosphatidylcholine, and stearic acid- and the thin-film hydration method for generating void and curcin-loaded HSLNs. (B) Conjugation strategy for RGD peptide and TF to create RGD-HSLNs, TF-HSLNs, and Dual (RGD+TF) HSLNs via NHS-EDC chemistry. (C) TEM image showing spherical, monodisperse nanoparticles with vesicular substructures. Scale bar = 200 nm. (D) Hydrodynamic diameter of various HSLN formulations measured by DLS, ranging from 147-192 nm. (E) Zeta potential showing uniformly negative surface charge across all formulations (-20 to -8 mV), with ligand conjugation causing slight surface potential shifts. (F) SDS-PAGE confirming TF conjugation by detection of a ~79 kDa band in TF-HSLNs and Dual-HSLNs. (M: Marker, TF-T: TF-HSLNs, D-T: Dual-HSLNs, T: Transferrin, * represents 75 kDa M.W.) (G) MALDI-TOF mass spectrometry confirming RGD conjugation with a 588.35 m/z peak (arrow), indicative of successful peptide attachment. (H) SDS-PAGE validating curcin encapsulation, showing a 28 kDa band in curcin-loaded HSLNs. (M: Marker, C: Curcin, N-C: Curcin-HSLN, N: Void-HSLN, * represents 25 kDa M.W.). (I) In vitro release profile of curcin from HSLNs under acidic (pH 4.0, 6.5) and physiological (pH 7.4) conditions over 96 h. The biphasic profile includes an initial burst followed by sustained release; significantly higher release was observed under acidic conditions, simulating tumor microenvironments. Data in (D-E, I) are presented as mean ± SD (n = 3 independent replicates). Statistical analysis: one-way ANOVA with Tukey's post hoc test; p < 0.05 (*), p < 0.01 (**).

Article Snippet: HSLNs composed of DSPE-PEG(2000) Amine [1,2-distearoyl- sn -glycero-3-phosphoethanolamine-N-[amino(polyethylene glycol)-2000] (ammonium salt) (Avanti Polar Lipids, USA), stearic acid, and lecithin (both from Sigma-Aldrich, USA) were synthesized via a modified lipid co-acervation method .

Techniques: Conjugation Assay, Zeta Potential Analyzer, SDS Page, Marker, Mass Spectrometry, Encapsulation, In Vitro

Journal: Theranostics

Article Title: Dual-ligand curcin-loaded hybrid solid lipid nanoparticles achieve durable gliosarcoma remission while preserving neuro-behavioral function

doi: 10.7150/thno.123534

Figure Lengend Snippet: Synthesis, physicochemical characterization, and pH-responsive release profile of curcin-loaded dual-targeted hybrid solid lipid nanoparticles (HSLNs). (A) Schematic illustration of lipid components-DSPE-PEG(2000)-amine, phosphatidylcholine, and stearic acid- and the thin-film hydration method for generating void and curcin-loaded HSLNs. (B) Conjugation strategy for RGD peptide and TF to create RGD-HSLNs, TF-HSLNs, and Dual (RGD+TF) HSLNs via NHS-EDC chemistry. (C) TEM image showing spherical, monodisperse nanoparticles with vesicular substructures. Scale bar = 200 nm. (D) Hydrodynamic diameter of various HSLN formulations measured by DLS, ranging from 147-192 nm. (E) Zeta potential showing uniformly negative surface charge across all formulations (-20 to -8 mV), with ligand conjugation causing slight surface potential shifts. (F) SDS-PAGE confirming TF conjugation by detection of a ~79 kDa band in TF-HSLNs and Dual-HSLNs. (M: Marker, TF-T: TF-HSLNs, D-T: Dual-HSLNs, T: Transferrin, * represents 75 kDa M.W.) (G) MALDI-TOF mass spectrometry confirming RGD conjugation with a 588.35 m/z peak (arrow), indicative of successful peptide attachment. (H) SDS-PAGE validating curcin encapsulation, showing a 28 kDa band in curcin-loaded HSLNs. (M: Marker, C: Curcin, N-C: Curcin-HSLN, N: Void-HSLN, * represents 25 kDa M.W.). (I) In vitro release profile of curcin from HSLNs under acidic (pH 4.0, 6.5) and physiological (pH 7.4) conditions over 96 h. The biphasic profile includes an initial burst followed by sustained release; significantly higher release was observed under acidic conditions, simulating tumor microenvironments. Data in (D-E, I) are presented as mean ± SD (n = 3 independent replicates). Statistical analysis: one-way ANOVA with Tukey's post hoc test; p < 0.05 (*), p < 0.01 (**).

Article Snippet: HSLNs composed of DSPE-PEG(2000) Amine [1,2-distearoyl- sn -glycero-3-phosphoethanolamine-N-[amino(polyethylene glycol)-2000] (ammonium salt) (Avanti Polar Lipids, USA), stearic acid, and lecithin (both from Sigma-Aldrich, USA) were synthesized via a modified lipid co-acervation method .

Techniques: Conjugation Assay, Zeta Potential Analyzer, SDS Page, Marker, Mass Spectrometry, Encapsulation, In Vitro